FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2955313
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG4650.012.90455
TGGGGGG3150.012.8584125
GTATTAG6850.012.7026281
TAGTCAG3950.012.1512489
GGTAGTC4900.011.632037
TATTAGG8250.011.2736492
TAGGGGG2301.3133103E-911.0878644
GCCGCGT2504.5292836E-1010.80070125
GTATAGA8350.010.6003871
AGGGGGG3550.010.5644235
ATTAGGT7150.010.4903693
GTAGTCA5300.010.1881338
CGCCGCG2701.7280399E-910.00014124
TTAGGTA7400.09.9332454
AGTCAGA5250.09.71376510
CTAATAC7850.09.5549213
CCTAGCG2054.168489E-69.51297430
TAATACT15650.09.393744
GTATAAA11700.09.36037351
TCTTATA9650.09.3271882
Produced by FastQC (version 0.11.2)