FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3438641
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCGG750.001019200213.99903713
TGGGGGG3550.012.6766595
GCAGCGT3450.012.1766171
TCCGAGC3000.011.99917513
CCGAGCC3000.011.49920914
TAGGTAG4850.010.5159645
TAGGGGG2651.2496457E-910.1891634
GCGCGGT1200.00295774439.99945611
GGTAGTC4500.09.9993117
GCGTCAG4250.09.8828224
TAGTCAG4300.09.7669119
TTGGGGG3900.09.6158414
GTGCGCG1250.00414565959.5994799
TAGACGG2352.3651592E-79.57492355
CTCCGAG3950.09.493155512
GTATTAG9050.09.4496121
GCCCACG4850.09.27771218
TATACTG14350.09.1990445
CCTAGCG2301.8149512E-69.131430
CAGCGTC4650.09.0329492
Produced by FastQC (version 0.11.2)