FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences694725
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGCA400.00651021718.7488779
GGATAGG550.002098329116.3661886
TAGGGAC550.00209933916.3650113
ACTGCGT550.002101359416.3626548
CGAGGCT600.003748038615.00233929
GTATAGA2300.013.6987751
CTAATAC1701.9826984E-913.2364063
CTGATCG800.001656006913.1242139
TATACAG2153.274181E-1112.5600995
AGAGCGG850.00261164112.35220058
TAGGAGT1006.434865E-412.0018724
AGTGAGG1006.446558E-411.99928110
TAGGACA1151.5743883E-411.7409624
CACACTG900.00399969211.6684865
TGATCGA900.004006151611.66596610
ACTGTGC900.004006151611.6659668
GTCTAAT1303.8294587E-511.5410931
GTATAGG1459.291873E-611.3819061
TAAGGTA1202.3381828E-411.2517554
TATAGGA1755.453858E-711.1437932
Produced by FastQC (version 0.11.2)