FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1482925
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG3650.014.3862771
CAGCGCA1004.2753923E-513.4989099
ACAGCGC1151.094367E-513.0424238
GTACTAA2102.1827873E-1112.8595711
TAGTCAG2204.9112714E-1112.2717339
GGCGCCG950.005997093411.052855525
GGACTGC1100.001431656710.9104166
ACAGTGT2651.09139364E-1010.7538478
GTCTTAG1704.667658E-610.5902361
TACGGAC1000.00877119710.5012753
GCTACGC1000.0087733910.5009229
GCCCGTA1000.00877777810.50021326
TAATACT5600.010.4476984
GTATAAC3750.010.4019641
GGTAGCC1756.6190987E-610.2848838
AATACAG4900.010.1032675
CTAGACC1501.6416945E-410.0012144
TAGGTAG2251.2517012E-710.0012145
CTAATAC3451.8189894E-1210.0012143
CCTGCGT1356.9563766E-410.00020225
Produced by FastQC (version 0.11.2)