FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016625
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG1503.0377123E-1015.0018955
TCTACAC2000.015.0018953
GGGGTGT600.003750371315.0018946
TAGGGGG600.003750371315.0018944
TCCCGCT851.6348125E-414.11595711
CGGACTC750.001016454214.00245730
AGGGGGG1251.7733255E-613.2016675
CTAACAC1404.4977241E-712.8587673
ATAGCGT1302.7535934E-612.6939116
CCGCCCG954.190907E-412.6300679
CCTAATA1702.8194336E-812.3545012
GTATGAG1101.0385728E-412.2779021
GGACCGA1101.0414403E-412.2742786
TATAGGG1406.303E-611.7872032
TAGGACC1459.314223E-611.3807474
ATTAGGT2259.076757E-1011.3347643
ATAAGCC1202.3408567E-411.2514213
TTACGCT1202.3408567E-411.2514214
TCCTATA1602.25646E-611.251422
TAGTCAG1202.3462053E-411.2486539
Produced by FastQC (version 0.11.2)