FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2810981
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG3000.014.5009635
CTAATAC8900.013.4840423
TAGGTAG4300.013.2566945
CTAGGCA2900.012.92982310
CGATCAC2600.012.69247424
GGTAGTC4150.012.6496427
TAGGGGG1907.730705E-1012.6324194
CCTAATA8400.012.1436642
TAATACT12300.011.4641764
TAGTCAG4450.011.4596019
GTATTAG7150.011.1206081
TCTTATA7650.010.9811212
TATACTG9700.010.8254615
GTATAGA8950.010.7279231
ACTATGC9400.010.6904888
TTAGGTA5900.010.6786764
CTATGCA6750.010.6656689
CCGAGCC3101.8189894E-1210.64473314
GCCGCGT2402.6539055E-910.62570625
CTCCGAG3550.010.56276712
Produced by FastQC (version 0.11.2)