FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9266975
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG6550.012.8251334
TGGGGGG10000.011.4006265
TAGGTAG11250.010.5339135
GTATTAG24150.010.4365961
AGGGGGG10200.010.2946845
CTAATAC26950.010.1303733
ACTATGC23500.09.44605358
TACACCG9200.09.293995
TAATACT39950.09.0868594
TCTATAC25400.09.0358823
TAGACAG15050.08.9705935
GTATATA57800.08.8250631
CTATGCA21100.08.8144639
GTATAGA25600.08.7905891
CTACACA13850.08.7730464
CCTAATA27550.08.711962
GTATTAT41900.08.7007961
GTCTTAG11050.08.6892921
CCGTGCG1901.8211604E-48.68351659
GCCGCGT6450.08.6053125
Produced by FastQC (version 0.11.2)