FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6441027
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG7200.014.1679915
TAGGGGG4450.011.1246354
AGGGGGG7150.010.70035
GTATTAG16850.010.5062481
GTGTCCG2803.245077E-99.6423369
TATACGG16250.09.4162655
TAATACT34100.09.4143694
ATTATAC30950.09.4030683
CTAATAC18050.09.3082763
TTATACT27350.09.2147554
TTGGGGG7750.09.0976244
CAGTGCG3306.1299943E-109.0904179
GTATTAA20150.08.9345311
ACCGTGC2905.8649675E-88.7926278
CCCGGGG1550.00245794868.70981427
TCTATAC17650.08.4993113
ACGGTGT12450.08.4332798
TATACTG24550.08.432565
GTATTAT34600.08.4118431
GTATATA58500.08.386051
Produced by FastQC (version 0.11.2)