FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1783646
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG1605.0931703E-1115.0014585
TCTAGAC2550.012.3537953
TGCAGTC1702.8303475E-812.3527567
GCGTCAG1951.1641532E-912.3088884
GTGCGAC2401.8189894E-1111.87515529
CTGATCG900.004009012611.6664929
TAGACTG1459.328238E-611.3804165
TGCGACG2900.011.37945830
GTATTAG4650.010.9688081
ACTATGC3700.010.9457838
GAGCGCA1653.2744283E-610.9089289
TAGGTAG3200.010.7822985
CTAGGAC1304.914038E-410.3853343
ATCGTGC1304.91785E-410.384468
TACAGGC3650.010.2738197
AGGACAG2352.1620508E-810.2137585
ACACCGT2701.722583E-910.0009726
GGTGCGA3001.0004442E-1010.00013228
CTTAGAC2301.7268576E-79.7832853
TAGACAG2803.2268872E-99.6437945
Produced by FastQC (version 0.11.2)