FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12350214
Sequences flagged as poor quality0
Sequence length36
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG11950.012.30168155
TAGGGGG10150.012.1186484
AGGGGGG11700.011.6670715
CTGGGCG2157.266972E-79.7668719
AGACCGC3306.002665E-119.5458256
ACCGTGC4100.09.1458078
GTATATA77900.08.9548231
GTATAGG17650.08.6695591
CTTACAC19600.08.2655593
CCGTGCA3654.0763553E-98.2186989
ACAGTGC12600.08.2138068
TATACTG37500.08.2002855
TCTATAC34100.08.182073
TATACAG40750.08.1720635
TTAGGGG8300.08.1327793
GTCTTAT21900.08.0146261
TATAGGG14800.08.0070342
TAGACGG4905.456968E-127.959465
CTTATAC34200.07.938843
GTATTAA36350.07.92388731
Produced by FastQC (version 0.11.2)