FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5785703
Sequences flagged as poor quality0
Sequence length36
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG6000.012.0005235
GGGGCCG1151.5817763E-411.73811911
CGGGGGG1202.346272E-411.2504895
AGGGGGG6550.010.534815
CTATACT11350.09.6479974
ATTATAC26000.09.519483
GGCGCCG2054.1694057E-69.513267525
TATACAG18800.09.4950935
TAGGGGG5300.09.3400294
TAGACTG5250.09.1432545
TTATACT21850.09.1308334
TATACTG16850.09.0805145
GTATTAG19250.09.0398241
GCGCCGA2003.2182954E-59.00101426
CTTATAC12900.08.9537233
GCACGCT1550.00246014268.70892711
TAATACT29400.08.6228244
TATATAC32550.08.6177383
TAGGCCG1757.70359E-48.5718025
TATTATA47500.08.526542
Produced by FastQC (version 0.11.2)