FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2838522
Sequences flagged as poor quality0
Sequence length36
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTATA5900.011.95021252
CTCTTAT5450.011.8373721
GTATAAT3950.011.0149941
TATAATA4400.010.9100582
GGACCGT2651.09139364E-1010.755866
TAGGGGT1602.7745471E-510.3135974
CCCTTAT2352.1607775E-810.21493151
GTATAAG3700.010.1372831
TCTATAC4300.010.1171763
TAGACTG3501.8189894E-129.8581915
TTATACA8550.09.6501484
TAGGGCT2701.830449E-89.4454484
TAAAGGT4450.09.4392054
GACCTAT1603.145187E-49.3759976
TCCTATA2752.4687324E-89.2735492
CTAGACT4100.09.1473134
TAGACAG4550.08.9020455
TATATAA5600.08.8400692
GGTATAT4251.8189894E-128.82541
CTTATAC8450.08.6989973
Produced by FastQC (version 0.11.2)