FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301972
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACG400.00649363318.7530022
CTGTCTA550.002099217116.3608369
ATACTGG809.701056E-515.0024026
CAATGCT706.0112943E-415.0024024
TTGTACT650.006364924413.8483714
AGAGACG650.006364924413.8483715
CAATACA1056.707698E-512.8592014
ATACAGA954.1659203E-412.6336016
TTTATCC1006.416086E-412.0019223
CACGAGA950.005972148411.05440122
TAATACT1551.9373501E-510.6468654
ACAATAC1000.00874155810.5016813
AATACAG1000.00874155810.5016815
GAGACAG1000.00875230310.49994318
CTTATAC1150.002064386110.4364542
TATCCTT1150.002064386110.4364545
TTCCAAC1304.8995885E-410.38283811
ATTATAC1200.002939209310.00163
TTATTCA1300.00569219249.2322474
GTATTAG1350.0077605938.8903131
Produced by FastQC (version 0.11.2)