FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2260845
Sequences flagged as poor quality0
Sequence length36
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGGG755.6247867E-515.9999773
AGGGGGG1251.1340853E-714.4002975
CTTAGCC1302.7617807E-612.6922893
TAATACG1406.3215793E-611.7856974
GTGTAGA4400.09.8876611
TATAGTA2905.693437E-109.8275722
CCCTACA2157.250055E-79.7674272
TGGGGGG1705.4062468E-59.7060825
ATAGGAC1409.7171555E-49.6428433
TAATACC3001.0513759E-99.4999874
CTTATAC4900.09.4897823
TATACAC4800.09.3749863
TAAGACT2454.328449E-79.18366054
GGACTGC1801.0098179E-49.1668566
ATAATAC4100.09.1463283
CTCTTAG1654.2784534E-49.0921021
CTTCTAA2003.2148702E-59.0011821
TGACCTA1500.00182378349.0001865
GTAATAC3001.0581061E-88.9999883
TAGGGGT1500.00182415048.9999884
Produced by FastQC (version 0.11.2)