##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	HK5NHBGXX_combined_n01_ntr_6.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27
Sequences flagged as poor quality	0
Sequence length	36
%GC	39
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.59259259259259	0.0	0.0	0.0	0.0	0.0
2	31.333333333333332	0.0	0.0	0.0	0.0	0.0
3	32.0	0.0	0.0	0.0	0.0	0.0
4	32.0	0.0	0.0	0.0	0.0	0.0
5	32.0	0.0	0.0	0.0	0.0	0.0
6	33.74074074074074	0.0	0.0	0.0	0.0	0.0
7	34.629629629629626	0.0	0.0	0.0	0.0	0.0
8	35.03703703703704	0.0	0.0	0.0	0.0	0.0
9	34.074074074074076	0.0	0.0	0.0	0.0	0.0
10	34.888888888888886	0.0	0.0	0.0	0.0	0.0
11	35.7037037037037	0.0	0.0	0.0	0.0	0.0
12	34.074074074074076	0.0	0.0	0.0	0.0	0.0
13	34.44444444444444	0.0	0.0	0.0	0.0	0.0
14	34.592592592592595	0.0	0.0	0.0	0.0	0.0
15	34.851851851851855	0.0	0.0	0.0	0.0	0.0
16	35.074074074074076	0.0	0.0	0.0	0.0	0.0
17	34.888888888888886	0.0	0.0	0.0	0.0	0.0
18	34.370370370370374	0.0	0.0	0.0	0.0	0.0
19	36.0	0.0	0.0	0.0	0.0	0.0
20	35.51851851851852	0.0	0.0	0.0	0.0	0.0
21	34.888888888888886	0.0	0.0	0.0	0.0	0.0
22	35.51851851851852	0.0	0.0	0.0	0.0	0.0
23	34.2962962962963	0.0	0.0	0.0	0.0	0.0
24	35.7037037037037	0.0	0.0	0.0	0.0	0.0
25	33.925925925925924	0.0	0.0	0.0	0.0	0.0
26	34.74074074074074	0.0	0.0	0.0	0.0	0.0
27	35.074074074074076	0.0	0.0	0.0	0.0	0.0
28	35.7037037037037	0.0	0.0	0.0	0.0	0.0
29	33.111111111111114	0.0	0.0	0.0	0.0	0.0
30	34.333333333333336	0.0	0.0	0.0	0.0	0.0
31	33.48148148148148	0.0	0.0	0.0	0.0	0.0
32	34.18518518518518	0.0	0.0	0.0	0.0	0.0
33	35.851851851851855	0.0	0.0	0.0	0.0	0.0
34	34.22222222222222	0.0	0.0	0.0	0.0	0.0
35	35.7037037037037	0.0	0.0	0.0	0.0	0.0
36	34.074074074074076	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
21610	1	0.0
21610	2	0.0
21610	3	0.0
21610	4	0.0
21610	5	0.0
21610	6	4.5
21610	7	0.0
21610	8	0.0
21610	9	2.0
21610	10	0.0
21610	11	0.0
21610	12	-2.0
21610	13	2.0
21610	14	-9.0
21610	15	0.0
21610	16	0.0
21610	17	0.0
21610	18	0.0
21610	19	0.0
21610	20	0.0
21610	21	2.0
21610	22	0.0
21610	23	0.0
21610	24	0.0
21610	25	0.0
21610	26	0.0
21610	27	0.0
21610	28	0.0
21610	29	0.0
21610	30	0.0
21610	31	0.0
21610	32	7.5
21610	33	0.0
21610	34	-11.0
21610	35	0.0
21610	36	-2.0
21308	1	0.0
21308	2	0.0
21308	3	0.0
21308	4	0.0
21308	5	0.0
21308	6	-4.5
21308	7	0.0
21308	8	0.0
21308	9	-2.0
21308	10	0.0
21308	11	0.0
21308	12	2.0
21308	13	-2.0
21308	14	9.0
21308	15	0.0
21308	16	0.0
21308	17	0.0
21308	18	0.0
21308	19	0.0
21308	20	0.0
21308	21	-2.0
21308	22	0.0
21308	23	0.0
21308	24	0.0
21308	25	0.0
21308	26	0.0
21308	27	0.0
21308	28	0.0
21308	29	0.0
21308	30	0.0
21308	31	0.0
21308	32	-7.5
21308	33	0.0
21308	34	11.0
21308	35	0.0
21308	36	2.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
30	1.0
31	1.0
32	1.0
33	6.0
34	5.0
35	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.925925925925924	22.22222222222222	18.51851851851852	33.33333333333333
2	7.4074074074074066	22.22222222222222	48.148148148148145	22.22222222222222
3	14.814814814814813	14.814814814814813	48.148148148148145	22.22222222222222
4	25.925925925925924	7.4074074074074066	55.55555555555556	11.11111111111111
5	7.4074074074074066	29.629629629629626	48.148148148148145	14.814814814814813
6	33.33333333333333	51.85185185185185	0.0	14.814814814814813
7	18.51851851851852	40.74074074074074	25.925925925925924	14.814814814814813
8	18.51851851851852	22.22222222222222	25.925925925925924	33.33333333333333
9	11.11111111111111	25.925925925925924	40.74074074074074	22.22222222222222
10	7.4074074074074066	29.629629629629626	37.03703703703704	25.925925925925924
11	29.629629629629626	22.22222222222222	44.44444444444444	3.7037037037037033
12	18.51851851851852	14.814814814814813	33.33333333333333	33.33333333333333
13	11.11111111111111	25.925925925925924	37.03703703703704	25.925925925925924
14	18.51851851851852	33.33333333333333	14.814814814814813	33.33333333333333
15	7.4074074074074066	44.44444444444444	18.51851851851852	29.629629629629626
16	25.925925925925924	29.629629629629626	22.22222222222222	22.22222222222222
17	18.51851851851852	40.74074074074074	22.22222222222222	18.51851851851852
18	18.51851851851852	29.629629629629626	29.629629629629626	22.22222222222222
19	22.22222222222222	37.03703703703704	22.22222222222222	18.51851851851852
20	18.51851851851852	37.03703703703704	25.925925925925924	18.51851851851852
21	25.925925925925924	22.22222222222222	40.74074074074074	11.11111111111111
22	25.925925925925924	29.629629629629626	29.629629629629626	14.814814814814813
23	11.11111111111111	33.33333333333333	33.33333333333333	22.22222222222222
24	18.51851851851852	37.03703703703704	22.22222222222222	22.22222222222222
25	22.22222222222222	29.629629629629626	37.03703703703704	11.11111111111111
26	22.22222222222222	29.629629629629626	29.629629629629626	18.51851851851852
27	22.22222222222222	18.51851851851852	40.74074074074074	18.51851851851852
28	22.22222222222222	33.33333333333333	33.33333333333333	11.11111111111111
29	14.814814814814813	37.03703703703704	29.629629629629626	18.51851851851852
30	22.22222222222222	25.925925925925924	29.629629629629626	22.22222222222222
31	11.11111111111111	48.148148148148145	22.22222222222222	18.51851851851852
32	37.03703703703704	25.925925925925924	7.4074074074074066	29.629629629629626
33	25.925925925925924	33.33333333333333	22.22222222222222	18.51851851851852
34	25.925925925925924	29.629629629629626	25.925925925925924	18.51851851851852
35	25.925925925925924	48.148148148148145	11.11111111111111	14.814814814814813
36	11.11111111111111	40.74074074074074	40.74074074074074	7.4074074074074066
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	1.0
17	1.0
18	0.5
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.5
25	1.0
26	1.0
27	1.0
28	1.0
29	1.0
30	1.0
31	1.0
32	1.5
33	2.0
34	2.0
35	4.5
36	7.0
37	7.0
38	4.5
39	2.0
40	3.0
41	4.0
42	4.0
43	2.0
44	0.0
45	0.0
46	2.0
47	4.0
48	4.0
49	2.5
50	1.0
51	1.0
52	1.0
53	1.0
54	1.5
55	2.0
56	2.0
57	1.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
36	27.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	100.0
#Duplication Level	Percentage of deduplicated	Percentage of total
1	100.0	100.0
2	0.0	0.0
3	0.0	0.0
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTATTGTCCAGTTCTGATGAGGTGTTTGAAAAGGGA	1	3.7037037037037033	No Hit
TATGAAGTTCGCCATACGAAGGCCACTCAGCCCAGT	1	3.7037037037037033	No Hit
GACGTATCGTGCCGATCCGTTGATGCGTCCTGAGAT	1	3.7037037037037033	No Hit
ATTGTCATATTCATGGCGGATAATTCTTTTAACGCG	1	3.7037037037037033	No Hit
TTATCGCCCATCTAGCAATTTTTCCTCATTTGACAA	1	3.7037037037037033	No Hit
GGGTAGGTTATGAAAAACACTTCAATGCATTGAAAT	1	3.7037037037037033	No Hit
GCTGAAGAGTCTTTCTCAATTGCATGGCCGCTATGA	1	3.7037037037037033	No Hit
GGCTCAACAAGCCACCGATGGCTTGTCTCAGGAACT	1	3.7037037037037033	No Hit
ACTAGCACTATTACTCGTTGAATATATAAGGGACAT	1	3.7037037037037033	No Hit
TCTTCACTTTAGATAAATTGCATACTTGATACATAT	1	3.7037037037037033	No Hit
CTTGAAATTATTCGCGTGCATCAATGGGTCGCTGTC	1	3.7037037037037033	No Hit
AATCTAAAATGGCAAAAAAGGCAAAATTGGAAAAGA	1	3.7037037037037033	No Hit
CTTTCAAGAATCACCTAAAGGCTTTGCATTTCGAGT	1	3.7037037037037033	No Hit
CTCTTAACTTGTTCAAGTCAATGGAATGTACCTGAT	1	3.7037037037037033	No Hit
ATTTTGGCATGCTGAAAAAAAAAAAAAAAAATTTAT	1	3.7037037037037033	No Hit
TTACAATACCTATCCAACTCTGGGGATGGGAGTAAA	1	3.7037037037037033	No Hit
CTCGAGAGATTTTAACTTAATACACAAAACAACACA	1	3.7037037037037033	No Hit
GCTTAATCTTTGAGACAAGCATATGACTACTGGCAG	1	3.7037037037037033	No Hit
CATTTGTGTCTCTACTTCTTGTTCGCCTGGAGGGAA	1	3.7037037037037033	No Hit
CTTTTCAACCAAGAATTCAAAATCTGGAGAACCTAG	1	3.7037037037037033	No Hit
GTCTTCCTTAGTTGTCTTGTGGGAGTTACCAACGGT	1	3.7037037037037033	No Hit
ACCTTGTCTGAGTCCGGGCCCTTGTGAATAAAGTCA	1	3.7037037037037033	No Hit
GTGGTAATCCATGTCGGAGACAATAAGTTTTGGCAA	1	3.7037037037037033	No Hit
CCTTTACGGTTCCCTGAGATTGAATTAGTTCCTTTA	1	3.7037037037037033	No Hit
CAGCTATATGAAGAATCTCTATCCAGTTCCAGTTTT	1	3.7037037037037033	No Hit
TTGAAGGACCTTCCAAACAATTACATTTATACGCAC	1	3.7037037037037033	No Hit
AAATGGAGACAAACAGTTCCTACGTCAAAACAAAGA	1	3.7037037037037033	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10	0.0	0.0	0.0
11	0.0	0.0	0.0
12	0.0	0.0	0.0
13	0.0	0.0	0.0
14	0.0	0.0	0.0
15	0.0	0.0	0.0
16	0.0	0.0	0.0
17	0.0	0.0	0.0
18	0.0	0.0	0.0
19	0.0	0.0	0.0
20	0.0	0.0	0.0
21	0.0	0.0	0.0
22	0.0	0.0	0.0
23	0.0	0.0	0.0
24	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE