FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length36
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTGTCCAGTTCTGATGAGGTGTTTGAAAAGGGA13.7037037037037033No Hit
TATGAAGTTCGCCATACGAAGGCCACTCAGCCCAGT13.7037037037037033No Hit
GACGTATCGTGCCGATCCGTTGATGCGTCCTGAGAT13.7037037037037033No Hit
ATTGTCATATTCATGGCGGATAATTCTTTTAACGCG13.7037037037037033No Hit
TTATCGCCCATCTAGCAATTTTTCCTCATTTGACAA13.7037037037037033No Hit
GGGTAGGTTATGAAAAACACTTCAATGCATTGAAAT13.7037037037037033No Hit
GCTGAAGAGTCTTTCTCAATTGCATGGCCGCTATGA13.7037037037037033No Hit
GGCTCAACAAGCCACCGATGGCTTGTCTCAGGAACT13.7037037037037033No Hit
ACTAGCACTATTACTCGTTGAATATATAAGGGACAT13.7037037037037033No Hit
TCTTCACTTTAGATAAATTGCATACTTGATACATAT13.7037037037037033No Hit
CTTGAAATTATTCGCGTGCATCAATGGGTCGCTGTC13.7037037037037033No Hit
AATCTAAAATGGCAAAAAAGGCAAAATTGGAAAAGA13.7037037037037033No Hit
CTTTCAAGAATCACCTAAAGGCTTTGCATTTCGAGT13.7037037037037033No Hit
CTCTTAACTTGTTCAAGTCAATGGAATGTACCTGAT13.7037037037037033No Hit
ATTTTGGCATGCTGAAAAAAAAAAAAAAAAATTTAT13.7037037037037033No Hit
TTACAATACCTATCCAACTCTGGGGATGGGAGTAAA13.7037037037037033No Hit
CTCGAGAGATTTTAACTTAATACACAAAACAACACA13.7037037037037033No Hit
GCTTAATCTTTGAGACAAGCATATGACTACTGGCAG13.7037037037037033No Hit
CATTTGTGTCTCTACTTCTTGTTCGCCTGGAGGGAA13.7037037037037033No Hit
CTTTTCAACCAAGAATTCAAAATCTGGAGAACCTAG13.7037037037037033No Hit
GTCTTCCTTAGTTGTCTTGTGGGAGTTACCAACGGT13.7037037037037033No Hit
ACCTTGTCTGAGTCCGGGCCCTTGTGAATAAAGTCA13.7037037037037033No Hit
GTGGTAATCCATGTCGGAGACAATAAGTTTTGGCAA13.7037037037037033No Hit
CCTTTACGGTTCCCTGAGATTGAATTAGTTCCTTTA13.7037037037037033No Hit
CAGCTATATGAAGAATCTCTATCCAGTTCCAGTTTT13.7037037037037033No Hit
TTGAAGGACCTTCCAAACAATTACATTTATACGCAC13.7037037037037033No Hit
AAATGGAGACAAACAGTTCCTACGTCAAAACAAAGA13.7037037037037033No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)