FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380000
Sequences flagged as poor quality0
Sequence length36
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTATA400.006495090718.7537841
AGTAGGG400.006500028518.7513182
CTATGCT500.00110784318.0012634
TGTTAGA500.00110784318.0012632
GAGTAAG550.00209533416.3669381
GGATAGA653.423782E-416.1571041
TTCTAAC859.164771E-615.8834682
TGACCTG600.003746015515.0010535
CTAAACT600.003746015515.0010534
GTAAGAC706.0204783E-415.0010533
TAAGACT600.003746015515.0010534
AGTACTG706.0204783E-415.0010535
CTAATAC600.003746015515.0010533
GACACAC1104.18122E-714.9990812
TACTGAG600.003749297214.9990797
GAATAAG952.6209977E-514.2133941
TTAAAAC851.6279366E-414.1186383
TCTAACA750.001015205914.0009823
ATATTAG650.006367165613.8489481
CAAGAGG650.006372720513.8471254
Produced by FastQC (version 0.11.2)