FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131645
Sequences flagged as poor quality0
Sequence length36
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGAC300.001221421324.9977863
CTGAATT350.00299373221.4266714
TATGACT350.00299373221.426674
TTACACA504.566107E-520.9981384
TGTAGTA400.00648393418.7483372
ACACAGT550.002089604716.3621856
TACAAGG650.006350252313.8449272
Produced by FastQC (version 0.11.2)