FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3336685
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAC850.002616078412.35166913
CCGCGGA900.004013350711.66529112
GGACTGT2751.8189894E-1211.4559426
ACCGTGT2802.7284841E-1110.7131828
GTATTAA12250.010.4109921
CCGGGCA1304.9242494E-410.3835469
TAGCCTG3051.2732926E-1110.3291275
TATACTG13700.010.2932255
AGGGGTG2209.030191E-810.2285195
ACCTAGG1501.6406005E-410.0027181
CACCGCG1356.9691107E-49.99897110
CCCTTAG3650.09.8656951
GCGTCAG1851.28026295E-59.7309164
GCCCACG1851.2834536E-59.72887418
CTTACAC4650.09.6784533
ATACTGT10750.09.6290816
ACTATGC3603.6379788E-129.5823478
CTGGGCA2209.942141E-79.5444739
TTATACT18950.09.4998394
GTAGGAC2854.3837645E-99.4746973
Produced by FastQC (version 0.11.2)