FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359670
Sequences flagged as poor quality0
Sequence length36
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATG4840.13456779825951568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCATG953.8253347E-918.94561230
ACACCGT400.00650026118.7508686
GTATTAA600.00374608915.0006951
TCTTATG600.00374608915.0006952
GTACTAG706.020457E-415.0006951
TAGATAG706.020457E-415.0006955
CTCTTAT1503.0195224E-1015.0006941
TATGGAC650.006372848513.8467963
TAATACA902.642982E-413.3339514
ATAAGAC800.001651980713.1256083
ATAATAC1151.087469E-513.0440833
TAGGCAT1302.7463066E-612.69113229
CCGAGCC1456.8478766E-712.41436914
TTATATA850.00260533912.3535152
ACTAGGC1252.5645491E-511.99888727
TCCGAGC1406.2768813E-611.78626113
TATATTA900.003996554311.6672082
GACTAGG1303.8245824E-511.53899726
GTATTAC1059.6787E-411.4291011
CGAGCCC1459.2758855E-611.37983815
Produced by FastQC (version 0.11.2)