FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7450838
Sequences flagged as poor quality0
Sequence length36
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG3650.011.0963754
AGGGGGG5250.09.4289835
GTATATA21500.08.7919661
CCTTATA10250.08.6344652
TAGACAG8550.08.5968675
TTAGGAC3501.8535502E-98.5716883
GTTCTAG6600.08.4103041
CGCGTGA3101.7184902E-78.22533710
GTATAAA17550.08.2063121
TGGGGGG8050.08.1991155
GTATTAG10900.08.1204381
ACCGTGC10250.08.0483228
CCTAGGG2802.9501316E-68.0360112
GTATTAA11700.07.9498651
ACAGCGC7000.07.92811978
TTAGACT7400.07.90575084
GTCCTAC5700.07.8958761
TACCCTG7600.07.89508155
ATAATAC17100.07.89497473
TCTAGGG4955.456968E-127.8790792
Produced by FastQC (version 0.11.2)