FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4772844
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG5550.012.4329895
TAGGGGG3203.6379788E-1210.3129614
CTAATAC13650.010.220133
TGGGGGG5200.09.8081315
TAATACT22650.09.7355354
GTATTAG11000.09.6829611
GCGCACT3451.4551915E-119.564342511
ACTATGC11350.09.5147478
CTATGCA10450.09.3293889
GCGCGGT1300.0057300319.22992511
GCCGCGT3953.6379788E-129.11490425
GTATTAA15400.08.9620971
GGTAGTC6050.08.924997
ATCACAC8300.08.85665329
CGCCGCG3902.0008883E-118.84636424
GTCCTAA5650.08.7620961
TATTAGG13400.08.7316432
GGACGGC1550.0024570628.7100676
ACGGTGT8900.08.5948998
CGACGGG1757.711707E-48.5708248
Produced by FastQC (version 0.11.2)