FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7275185
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG3700.014.5962564
TGGGGGG7400.012.974455
CTAATAC19750.011.39370253
TAGGTAG10000.010.6512135
CCTAATA20150.010.5719232
TAATACT29000.010.1908154
ACTATGC19300.010.0252568
TATACTG22150.09.8205325
AGGGGGG8350.09.7017045
TACTATG21650.09.6298617
CTATGCA15700.09.4579859
CTAGGAC4950.09.3950093
GTATTAG15750.09.2400361
TATACCG12250.08.9397935
ACCTAAT19600.08.8794161
GTCCTAA9350.08.8253831
ATCACAC14000.08.67872429
GTATAGA18450.08.6196981
TAGACTG7150.08.6023785
GGTAGTC8750.08.3994557
Produced by FastQC (version 0.11.2)