FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4193643
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG6250.014.1613575
TAGGGGG2650.012.4538754
TATTAGG12050.011.3287512
GGCGCCG2402.2191671E-1011.25040725
TAGTCAG5600.011.2493339
TTAGGTA9750.011.0778524
TGGGGGG3550.010.9869675
TAGACTG4200.010.7153115
ACTATGC10550.010.6628768
GTATTAG11000.010.6383971
TAATACT16350.010.5513444
CTATGCA9150.010.4911819
CTTACAC5500.010.3645063
CTAATAC12500.010.2008553
GGTAGTC7000.010.0707127
TATACTG15100.09.7360325
CCTAATA12700.09.6858522
CTAGACT3655.456968E-129.45284754
CTAAGCA5250.09.4293624
TTATACT14100.09.3624874
Produced by FastQC (version 0.11.2)