FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5866734
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG6150.014.14735
CGGGGGG850.002613141412.3537775
GTATTAG14800.011.15111351
CTAATAC16050.010.8418543
TAGGGGG3500.010.715014
TAGTCAG6800.010.5874179
CCCCCGA2209.058931E-810.22674415
TAGGTAG7800.010.1929975
ACCGTGC3451.8189894E-129.9992278
ACTATGC16450.09.9384428
TATACTG21100.09.8821855
TAATACT26450.09.7549144
AGGGGGG5700.09.4743255
CCCCGAC2251.3498102E-69.33293116
GTATAGA16100.09.2256411
TTGGGGG5900.09.1531614
GGTAGTC9250.09.0804577
TATTAGG13900.09.0654382
ACCTAAT16450.09.0293521
CGCCGCG4500.08.99999524
Produced by FastQC (version 0.11.2)