FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2652100
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGGG1154.0810846E-815.652423
TGGGGGG2700.014.4446725
GTGAGCG900.004010670411.666199
GTATTAG7100.011.4094911
TAGGGGG1602.2639688E-611.2501764
TAGGTAG3051.8189894E-1210.8198425
GGGTAGG1951.895969E-710.7702121
CCGTCGT1551.9553934E-510.6457329
CTAATAC6950.010.57570653
ACTATGC5850.010.5123918
GTCTATA6600.010.4554981
TCCGAGC2905.2750693E-1110.34499113
ACAGTGC3800.010.2627398
CTGATCG2053.7793325E-710.24348459
TATACCG4900.010.10225
ACTGCGC1501.6454961E-49.9995918
TCTATAC8250.09.8183363
GCGTCAG2505.648144E-89.6001514
CCGAGCC2854.3892214E-99.47383314
GGCTGTG1603.1502542E-49.374447
Produced by FastQC (version 0.11.2)