##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	HK5NHBGXX_combined_n01_ntr_5.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29
Sequences flagged as poor quality	0
Sequence length	36
%GC	40
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.75862068965517	0.0	0.0	0.0	0.0	0.0
2	31.379310344827587	0.0	0.0	0.0	0.0	0.0
3	31.0	0.0	0.0	0.0	0.0	0.0
4	30.75862068965517	0.0	0.0	0.0	0.0	0.0
5	31.620689655172413	0.0	0.0	0.0	0.0	0.0
6	33.41379310344828	0.0	0.0	0.0	0.0	0.0
7	33.44827586206897	0.0	0.0	0.0	0.0	0.0
8	33.93103448275862	0.0	0.0	0.0	0.0	0.0
9	34.206896551724135	0.0	0.0	0.0	0.0	0.0
10	34.206896551724135	0.0	0.0	0.0	0.0	0.0
11	33.758620689655174	0.0	0.0	0.0	0.0	0.0
12	34.13793103448276	0.0	0.0	0.0	0.0	0.0
13	33.172413793103445	0.0	0.0	0.0	0.0	0.0
14	34.689655172413794	0.0	0.0	0.0	0.0	0.0
15	32.172413793103445	0.0	0.0	0.0	0.0	0.0
16	32.55172413793103	0.0	0.0	0.0	0.0	0.0
17	33.62068965517241	0.0	0.0	0.0	0.0	0.0
18	35.06896551724138	0.0	0.0	0.0	0.0	0.0
19	33.62068965517241	0.0	0.0	0.0	0.0	0.0
20	35.3448275862069	0.0	0.0	0.0	0.0	0.0
21	32.6551724137931	0.0	0.0	0.0	0.0	0.0
22	33.44827586206897	0.0	0.0	0.0	0.0	0.0
23	35.275862068965516	0.0	0.0	0.0	0.0	0.0
24	33.93103448275862	0.0	0.0	0.0	0.0	0.0
25	33.93103448275862	0.0	0.0	0.0	0.0	0.0
26	34.206896551724135	0.0	0.0	0.0	0.0	0.0
27	32.51724137931034	0.0	0.0	0.0	0.0	0.0
28	35.0	0.0	0.0	0.0	0.0	0.0
29	33.44827586206897	0.0	0.0	0.0	0.0	0.0
30	33.89655172413793	0.0	0.0	0.0	0.0	0.0
31	35.241379310344826	0.0	0.0	0.0	0.0	0.0
32	34.51724137931034	0.0	0.0	0.0	0.0	0.0
33	33.13793103448276	0.0	0.0	0.0	0.0	0.0
34	31.344827586206897	0.0	0.0	0.0	0.0	0.0
35	33.51724137931034	0.0	0.0	0.0	0.0	0.0
36	32.62068965517241	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
13404	1	0.0
13404	2	0.0
13404	3	0.0
13404	4	0.0
13404	5	0.0
13404	6	0.0
13404	7	11.0
13404	8	0.0
13404	9	0.0
13404	10	0.0
13404	11	0.0
13404	12	0.0
13404	13	0.0
13404	14	0.0
13404	15	-11.0
13404	16	0.0
13404	17	0.0
13404	18	7.5
13404	19	2.0
13404	20	2.0
13404	21	0.0
13404	22	0.0
13404	23	0.0
13404	24	0.0
13404	25	0.0
13404	26	0.0
13404	27	-11.0
13404	28	2.0
13404	29	0.0
13404	30	0.0
13404	31	0.0
13404	32	0.0
13404	33	0.0
13404	34	0.0
13404	35	0.0
13404	36	-7.5
12103	1	0.0
12103	2	0.0
12103	3	0.0
12103	4	0.0
12103	5	0.0
12103	6	0.0
12103	7	-11.0
12103	8	0.0
12103	9	0.0
12103	10	0.0
12103	11	0.0
12103	12	0.0
12103	13	0.0
12103	14	0.0
12103	15	11.0
12103	16	0.0
12103	17	0.0
12103	18	-7.5
12103	19	-2.0
12103	20	-2.0
12103	21	0.0
12103	22	0.0
12103	23	0.0
12103	24	0.0
12103	25	0.0
12103	26	0.0
12103	27	11.0
12103	28	-2.0
12103	29	0.0
12103	30	0.0
12103	31	0.0
12103	32	0.0
12103	33	0.0
12103	34	0.0
12103	35	0.0
12103	36	7.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	1.0
28	0.0
29	2.0
30	1.0
31	2.0
32	2.0
33	6.0
34	10.0
35	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.275862068965516	37.93103448275862	3.4482758620689653	10.344827586206897
2	17.24137931034483	24.137931034482758	41.37931034482759	17.24137931034483
3	6.896551724137931	51.724137931034484	17.24137931034483	24.137931034482758
4	13.793103448275861	20.689655172413794	44.827586206896555	20.689655172413794
5	10.344827586206897	44.827586206896555	37.93103448275862	6.896551724137931
6	37.93103448275862	34.48275862068966	20.689655172413794	6.896551724137931
7	34.48275862068966	24.137931034482758	31.03448275862069	10.344827586206897
8	24.137931034482758	37.93103448275862	24.137931034482758	13.793103448275861
9	24.137931034482758	17.24137931034483	17.24137931034483	41.37931034482759
10	17.24137931034483	34.48275862068966	41.37931034482759	6.896551724137931
11	20.689655172413794	34.48275862068966	34.48275862068966	10.344827586206897
12	17.24137931034483	34.48275862068966	34.48275862068966	13.793103448275861
13	20.689655172413794	27.586206896551722	31.03448275862069	20.689655172413794
14	31.03448275862069	20.689655172413794	17.24137931034483	31.03448275862069
15	24.137931034482758	31.03448275862069	34.48275862068966	10.344827586206897
16	20.689655172413794	31.03448275862069	31.03448275862069	17.24137931034483
17	24.137931034482758	24.137931034482758	31.03448275862069	20.689655172413794
18	13.793103448275861	48.275862068965516	20.689655172413794	17.24137931034483
19	27.586206896551722	27.586206896551722	24.137931034482758	20.689655172413794
20	20.689655172413794	20.689655172413794	31.03448275862069	27.586206896551722
21	17.24137931034483	24.137931034482758	24.137931034482758	34.48275862068966
22	24.137931034482758	20.689655172413794	34.48275862068966	20.689655172413794
23	10.344827586206897	41.37931034482759	20.689655172413794	27.586206896551722
24	24.137931034482758	34.48275862068966	20.689655172413794	20.689655172413794
25	13.793103448275861	44.827586206896555	34.48275862068966	6.896551724137931
26	20.689655172413794	31.03448275862069	27.586206896551722	20.689655172413794
27	10.344827586206897	37.93103448275862	24.137931034482758	27.586206896551722
28	24.137931034482758	17.24137931034483	13.793103448275861	44.827586206896555
29	31.03448275862069	24.137931034482758	20.689655172413794	24.137931034482758
30	13.793103448275861	34.48275862068966	34.48275862068966	17.24137931034483
31	20.689655172413794	17.24137931034483	44.827586206896555	17.24137931034483
32	20.689655172413794	27.586206896551722	41.37931034482759	10.344827586206897
33	13.793103448275861	24.137931034482758	41.37931034482759	20.689655172413794
34	20.689655172413794	41.37931034482759	20.689655172413794	17.24137931034483
35	20.689655172413794	31.03448275862069	34.48275862068966	13.793103448275861
36	10.344827586206897	31.03448275862069	34.48275862068966	24.137931034482758
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	0.5
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.5
25	1.0
26	1.0
27	1.0
28	1.0
29	1.0
30	1.0
31	1.0
32	1.5
33	2.0
34	2.0
35	3.0
36	4.0
37	4.0
38	4.5
39	5.0
40	4.0
41	3.0
42	3.0
43	3.0
44	3.0
45	3.0
46	3.0
47	3.0
48	3.0
49	2.5
50	2.0
51	1.5
52	1.0
53	1.0
54	1.5
55	2.0
56	2.0
57	1.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
36	29.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	100.0
#Duplication Level	Percentage of deduplicated	Percentage of total
1	100.0	100.0
2	0.0	0.0
3	0.0	0.0
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATCAAGCGTTGGGAGGCGCTTTATTACATTCTCAT	1	3.4482758620689653	No Hit
GTATGTGGGTCCTCATTCAGCTGGTCCACGTAACGC	1	3.4482758620689653	No Hit
GCCCAGAACGTCTAAGGGCATCACAGACCTGTTATT	1	3.4482758620689653	No Hit
AGTATGGGCTTTCGTTCACTGACGAGTACGGTGACC	1	3.4482758620689653	No Hit
GCTTAGGTGATAGCGGTAAAGTCAACCCGAAGTTTG	1	3.4482758620689653	No Hit
AAGTAGTCCAAAGGTTGAGGTCACTTCCGAAGATGA	1	3.4482758620689653	No Hit
CAATAGTGTGGTTGGTAACCAAATTTTCTAGGCGTT	1	3.4482758620689653	No Hit
ATCCTGACCTGTAATGAACTCTATATACTGCTGATA	1	3.4482758620689653	No Hit
GAATTATACAAAGTGTCTGTAGCATTAGGTTTAACG	1	3.4482758620689653	No Hit
ATTTTATATATATCACTTACCTTTTCTTTTTATAAA	1	3.4482758620689653	No Hit
ATATTTTTGGAAAGAACGCCACCACACGCACTTTAC	1	3.4482758620689653	No Hit
GTCTCTGGCTAAAAGCAAGTTATGGAGAGATTTGAT	1	3.4482758620689653	No Hit
TAAATAGAGTAGTTGAAACTAGATATCAATTGCACA	1	3.4482758620689653	No Hit
ATAATATATTATTGCTGATGGAATATTCTTCAACGT	1	3.4482758620689653	No Hit
GTTTTGCGGATTACTGCTGGAACGGCACACTAAGTA	1	3.4482758620689653	No Hit
ACAGAAAATAGAACAGTTGAAGCAGAAAAAGAGAAC	1	3.4482758620689653	No Hit
GGACATTGATATGTGCTAGCGTTAGACCATTCAATA	1	3.4482758620689653	No Hit
CAGGAAATATTGCCTATTTTCGTACAAGGTTACTTC	1	3.4482758620689653	No Hit
ATCGTGTTCCAAAGTAGAGTTCAGAATGTCGTCAAT	1	3.4482758620689653	No Hit
CCCTACCAGAGGGCTTTGTGCTGAAAACCAACGAGC	1	3.4482758620689653	No Hit
GTATAGGTAACACGCTTGAGCGCCATCCATTTTCAG	1	3.4482758620689653	No Hit
GGACACTGATTACTTAAAAAATATAGACGGATTCAT	1	3.4482758620689653	No Hit
GGAGGAACAAGTTAAACAATTTAGTGAGCAAAAGGA	1	3.4482758620689653	No Hit
GAAAGAAATGATTCTCGCTCCCACACATTACTTTTA	1	3.4482758620689653	No Hit
GTAACTGACTATAGGAGATACCACTGGTGCTGTAGT	1	3.4482758620689653	No Hit
GTATTGCACGGGCTCATCTTCGTCAATCAATTCAAT	1	3.4482758620689653	No Hit
GCCAAGGACTTCAAAAATTCGTCATGGTCCTGCTTT	1	3.4482758620689653	No Hit
AGACAAATCACTCCACCAACTAAGAACGGCCATGCC	1	3.4482758620689653	No Hit
ATCTTTGTCCTCTATTACAACGGATCTGGTGTTGTA	1	3.4482758620689653	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10	0.0	0.0	0.0
11	0.0	0.0	0.0
12	0.0	0.0	0.0
13	0.0	0.0	0.0
14	0.0	0.0	0.0
15	0.0	0.0	0.0
16	0.0	0.0	0.0
17	0.0	0.0	0.0
18	0.0	0.0	0.0
19	0.0	0.0	0.0
20	0.0	0.0	0.0
21	0.0	0.0	0.0
22	0.0	0.0	0.0
23	0.0	0.0	0.0
24	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE