FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29
Sequences flagged as poor quality0
Sequence length36
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCAAGCGTTGGGAGGCGCTTTATTACATTCTCAT13.4482758620689653No Hit
GTATGTGGGTCCTCATTCAGCTGGTCCACGTAACGC13.4482758620689653No Hit
GCCCAGAACGTCTAAGGGCATCACAGACCTGTTATT13.4482758620689653No Hit
AGTATGGGCTTTCGTTCACTGACGAGTACGGTGACC13.4482758620689653No Hit
GCTTAGGTGATAGCGGTAAAGTCAACCCGAAGTTTG13.4482758620689653No Hit
AAGTAGTCCAAAGGTTGAGGTCACTTCCGAAGATGA13.4482758620689653No Hit
CAATAGTGTGGTTGGTAACCAAATTTTCTAGGCGTT13.4482758620689653No Hit
ATCCTGACCTGTAATGAACTCTATATACTGCTGATA13.4482758620689653No Hit
GAATTATACAAAGTGTCTGTAGCATTAGGTTTAACG13.4482758620689653No Hit
ATTTTATATATATCACTTACCTTTTCTTTTTATAAA13.4482758620689653No Hit
ATATTTTTGGAAAGAACGCCACCACACGCACTTTAC13.4482758620689653No Hit
GTCTCTGGCTAAAAGCAAGTTATGGAGAGATTTGAT13.4482758620689653No Hit
TAAATAGAGTAGTTGAAACTAGATATCAATTGCACA13.4482758620689653No Hit
ATAATATATTATTGCTGATGGAATATTCTTCAACGT13.4482758620689653No Hit
GTTTTGCGGATTACTGCTGGAACGGCACACTAAGTA13.4482758620689653No Hit
ACAGAAAATAGAACAGTTGAAGCAGAAAAAGAGAAC13.4482758620689653No Hit
GGACATTGATATGTGCTAGCGTTAGACCATTCAATA13.4482758620689653No Hit
CAGGAAATATTGCCTATTTTCGTACAAGGTTACTTC13.4482758620689653No Hit
ATCGTGTTCCAAAGTAGAGTTCAGAATGTCGTCAAT13.4482758620689653No Hit
CCCTACCAGAGGGCTTTGTGCTGAAAACCAACGAGC13.4482758620689653No Hit
GTATAGGTAACACGCTTGAGCGCCATCCATTTTCAG13.4482758620689653No Hit
GGACACTGATTACTTAAAAAATATAGACGGATTCAT13.4482758620689653No Hit
GGAGGAACAAGTTAAACAATTTAGTGAGCAAAAGGA13.4482758620689653No Hit
GAAAGAAATGATTCTCGCTCCCACACATTACTTTTA13.4482758620689653No Hit
GTAACTGACTATAGGAGATACCACTGGTGCTGTAGT13.4482758620689653No Hit
GTATTGCACGGGCTCATCTTCGTCAATCAATTCAAT13.4482758620689653No Hit
GCCAAGGACTTCAAAAATTCGTCATGGTCCTGCTTT13.4482758620689653No Hit
AGACAAATCACTCCACCAACTAAGAACGGCCATGCC13.4482758620689653No Hit
ATCTTTGTCCTCTATTACAACGGATCTGGTGTTGTA13.4482758620689653No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)