FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4520214
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG1459.354093E-611.3785519
ACCGTGC2453.2014214E-1011.0196728
CTAATAC12700.09.9215863
CGTGCGC1409.7269355E-49.64221410
TAGGTAG5200.09.51964955
GTATAGA10400.09.3763451
GTCCTAG3254.5656634E-109.2320941
TATACTG14750.09.0512455
TAATACT18350.08.8287254
GGGGGGC2403.238085E-68.7494158
TAGGACT3952.5465852E-118.734564
TACACTG6700.08.7317275
GTCCAGA5500.08.7285241
TCTATAC9500.08.6844963
TAGACGG4003.45608E-118.6253795
TAGGGGG2803.215482E-78.5718054
TGGGGGG4201.0913936E-118.5718055
CCCCGAC2105.7179866E-58.57085616
CTATGCA8350.08.4425519
GTCTAAG6050.08.4309621
Produced by FastQC (version 0.11.2)