FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2864957
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG3400.012.7951825
TGGGGGG3100.012.5816925
GTATTAG8000.012.0018371
ACTGTGC3750.011.19936858
GGGTAGG2453.1832315E-1011.0220951
TCGCCCG1501.3607292E-511.00033924
GTCCCGT1100.001432819310.9099996
GTGCGAC3101.8189894E-1210.64567729
GCGGCCG1000.00878726110.49922514
ACACCGT3450.010.4356526
ACCGAGC1304.921683E-410.3840298
GTCTTAG3203.6379788E-1210.3140771
TGCGACG3350.010.29918530
CTGTGCG1756.6241264E-610.2851339
AGGGGGG1809.249012E-610.0008335
CTAGCCT2105.250695E-710.0008334
GGACCGT1356.956565E-410.0008326
GGTGCGA3305.456968E-1210.00048228
GCGCACT1200.0029579679.99926111
TAGGCTG1851.2806489E-59.7305395
Produced by FastQC (version 0.11.2)