FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2043778
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG2650.016.9804125
TGGGGGG1850.015.4047525
GCCGCGT1301.8277751E-713.84827825
CGCCGCG1404.5104207E-712.85754124
CCTAGCG1651.8600986E-812.73015930
GTATTAG5000.012.3006831
CTATGCA5050.012.1771069
GTATTAA5800.011.8972121
TAGTCAG3200.011.7176799
CGGGGTC900.004010177711.66617213
TTAGGTA4400.011.5904184
GTCCTAT3000.011.5006381
ACTATGC6050.011.4039168
ACCTCGC950.0060033911.0516218
CTAATAC7000.010.9281073
TTAGGAC2207.759809E-910.9086283
AGGGGGG2351.877197E-910.8506035
GTATTAT10550.010.6640981
TATACGG3850.010.5190345
ATTAGGT5000.010.4995553
Produced by FastQC (version 0.11.2)