FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3069136
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG3900.012.3085
GTATTAG8800.012.2730331
CCTAATA9150.011.6394452
TAATACT13450.011.2642214
CTAATAC9450.011.1112083
CCTAGCG2402.6557245E-910.62561230
CTCGCGC1000.00878636710.49940710
TATACTG12200.010.4510815
GTCTTAG3750.010.400261
GTATAGA9450.010.31771851
TAGTACT5050.010.0992624
TTAGGTA6700.010.0748794
CGGTCGA1200.00295764239.99943510
CTATGCA7200.09.7911149
GTATAAT9250.09.7299731
TGGGGGG2957.767085E-109.6612585
TAGTCAG4400.09.5449159
TATAATA17500.09.5143692
TTAGGCT3001.0531949E-99.5002374
TATTAGG8450.09.4083652
Produced by FastQC (version 0.11.2)