FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4176387
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGCG3200.012.65739130
GTATTAG11100.011.8939611
TAATACT20200.011.4365384
CTAGGAC3150.011.4294663
CTAATAC14100.011.383873
TAGGTAG6650.011.0536295
CTGTCCG1901.3285899E-711.0517769
CCTAATA14000.011.036712
ATCACAC9300.010.96873229
CTATGCA10500.010.7134569
ACGCCCG1551.958965E-510.64433811
AATACTA17100.010.6149935
GTATAGA11800.010.4255431
TCACACA10250.010.39118130
ACTATGC13850.010.28801258
TATACAG18800.010.2136875
GTACTAG7200.010.210111
TATACTG16400.010.1533555
CGTGAGC3401.8189894E-1210.14615212
TCTATAC14900.010.0679023
Produced by FastQC (version 0.11.2)