FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5364808
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG5400.013.6128085
CCGAGCC2505.2168616E-910.19908414
CTAATAC14300.09.966093
TAGACTG4400.09.8875955
TATACTG18000.09.7512145
ACCGCGC2403.2183743E-79.3742468
ACTATGC13650.09.3399088
TAGGTAG5800.09.3115055
CCCGACG1450.00134102029.30950912
TCTAAGC7800.09.2317483
CCGCGCA2601.03606E-79.2300269
GACCGCG2454.3375985E-79.1831057
CTAAGCA7200.09.1678094
GTCCTAA6100.09.1003431
GTATAGA16000.09.0957321
CTAGAAC9250.09.0820433
TAATACT24000.09.063634
TTGGGGG6150.09.0255154
TAGGACA7000.09.00112154
CAGTGCA7000.08.9992769
Produced by FastQC (version 0.11.2)