FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1968678
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATATATATACATGAGAAGAACGGCATAGTGCG32210.16361233274308953No Hit
TATATATACAGGCAACACGCAGATATAGGTGCGACG30280.1538087996107032No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGCA400.00651417718.74896812
TAGGTAG2850.017.8969355
GTATTAG5050.014.5562421
ACCGCGC650.006391585313.8450398
AGACCGC1106.906612E-613.6380396
GCGCACT1601.2141754E-813.12427811
TACACGG1507.3787305E-813.0015965
GTGCGAC6400.012.89188129
TAGTCAG3350.012.5363049
GGTGCGA6800.012.35414428
CGCTCGA850.002614979912.35194810
GGTTAGG1101.0424312E-412.2742351
AGCGCAC2600.012.11440910
TGCGACG6950.012.08750830
TTAGGTA4600.012.0666994
TGGGGGG2257.094059E-1112.0014725
CTAGGGT2257.094059E-1112.0014724
CCGGCTC1006.455553E-411.9993415
ACCGCGG900.004011227711.66572918
TCGGGGA1951.5337719E-811.53811914
Produced by FastQC (version 0.11.2)