FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3740031
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG4950.013.0314475
CGTCCGT1059.726744E-411.42758810
CGGTCGG950.006004731611.051681513
TGGGGGG3250.010.6163175
GTCGGGG1000.00878802310.49923715
CTTACAC6050.010.4139983
ACTATGC10200.010.2932328
TATACCG7600.010.2640585
TAGGGGG2651.2496457E-910.1895744
TCTATAC13600.010.1478143
GGCCAGG3001.0004442E-1010.0015471
ACCGTGC2105.2647374E-79.999148
GTATAGA11050.09.9110351
CTATGCA8350.09.8793899
AGGGGGG3650.09.863885
AGACCGC1851.2809129E-59.7305846
GTATTAG11750.09.7036291
CTAGGAC2209.919513E-79.5461653
ACCTAAT10900.09.49688151
TATACTG16950.09.3813555
Produced by FastQC (version 0.11.2)