FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6225183
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG5100.012.9424315
GTATTAG16350.011.2864051
ACGGTGT11850.011.1384418
GACCGCG1551.959095E-510.6444837
TGCGACG4800.010.31349930
TATACTG26350.010.1907415
CTAATAC18150.010.0008893
ACCGCGC2105.266138E-79.9992838
CGGTGTT11550.09.8694229
TACGGTG12950.09.8449337
TAATACT33050.09.5319384
TATACGG17500.09.5152085
AGGGGGG5450.09.35870555
CGCCGCG4250.09.1765524
ACTATGC16100.09.129788
CCTAATA23850.09.1203082
TCTTATA22550.09.1138932
TAGGGGG4450.09.1020064
AGCCCCG2157.653249E-69.06911710
TAGGTAG8750.08.915155
Produced by FastQC (version 0.11.2)