FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3623476
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGG800.001657180713.12544624
TAGGTAG5300.012.7361075
CCTAGCG2950.011.69628230
ACTATGC10200.011.4697138
CTAATAC12650.011.265053
TGGGGGG3550.010.9861375
GTATTAG9700.010.8257091
CACCCCG1408.1906954E-510.7134687
GGGGGGC1551.9587453E-510.6443498
CACGCGC1150.002076253810.43499521
CGCGCGC1150.002076253810.43499523
TAGTCAG4750.010.4202579
CTTACAC5150.010.1943823
CTAGGCA3850.010.12909710
ATCACAC8000.010.12604329
ACGGTGT7450.010.0663468
TAATACT18250.010.02764
TAGGGGG2105.2568794E-710.0002024
CTATGCA9250.09.8911369
CCTAATA12450.09.8798552
Produced by FastQC (version 0.11.2)