##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename HK5NHBGXX_combined_n01_ntr_4.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11 Sequences flagged as poor quality 0 Sequence length 36 %GC 33 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.0 0.0 0.0 0.0 0.0 0.0 2 32.0 0.0 0.0 0.0 0.0 0.0 3 30.363636363636363 0.0 0.0 0.0 0.0 0.0 4 30.363636363636363 0.0 0.0 0.0 0.0 0.0 5 30.363636363636363 0.0 0.0 0.0 0.0 0.0 6 34.09090909090909 0.0 0.0 0.0 0.0 0.0 7 34.0 0.0 0.0 0.0 0.0 0.0 8 34.54545454545455 0.0 0.0 0.0 0.0 0.0 9 32.90909090909091 0.0 0.0 0.0 0.0 0.0 10 32.81818181818182 0.0 0.0 0.0 0.0 0.0 11 35.63636363636363 0.0 0.0 0.0 0.0 0.0 12 33.27272727272727 0.0 0.0 0.0 0.0 0.0 13 33.63636363636363 0.0 0.0 0.0 0.0 0.0 14 36.0 0.0 0.0 0.0 0.0 0.0 15 35.18181818181818 0.0 0.0 0.0 0.0 0.0 16 33.63636363636363 0.0 0.0 0.0 0.0 0.0 17 33.27272727272727 0.0 0.0 0.0 0.0 0.0 18 34.90909090909091 0.0 0.0 0.0 0.0 0.0 19 32.0 0.0 0.0 0.0 0.0 0.0 20 36.0 0.0 0.0 0.0 0.0 0.0 21 35.27272727272727 0.0 0.0 0.0 0.0 0.0 22 36.0 0.0 0.0 0.0 0.0 0.0 23 35.27272727272727 0.0 0.0 0.0 0.0 0.0 24 33.90909090909091 0.0 0.0 0.0 0.0 0.0 25 32.0 0.0 0.0 0.0 0.0 0.0 26 36.0 0.0 0.0 0.0 0.0 0.0 27 35.27272727272727 0.0 0.0 0.0 0.0 0.0 28 34.81818181818182 0.0 0.0 0.0 0.0 0.0 29 34.0 0.0 0.0 0.0 0.0 0.0 30 34.81818181818182 0.0 0.0 0.0 0.0 0.0 31 34.0 0.0 0.0 0.0 0.0 0.0 32 33.63636363636363 0.0 0.0 0.0 0.0 0.0 33 33.63636363636363 0.0 0.0 0.0 0.0 0.0 34 31.636363636363637 0.0 0.0 0.0 0.0 0.0 35 36.0 0.0 0.0 0.0 0.0 0.0 36 30.272727272727273 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 12409 1 0.0 12409 2 0.0 12409 3 0.0 12409 4 0.0 12409 5 0.0 12409 6 0.0 12409 7 0.0 12409 8 0.0 12409 9 0.0 12409 10 0.0 12409 11 0.0 12409 12 0.0 12409 13 0.0 12409 14 0.0 12409 15 0.0 12409 16 0.0 12409 17 0.0 12409 18 0.0 12409 19 0.0 12409 20 0.0 12409 21 0.0 12409 22 0.0 12409 23 0.0 12409 24 0.0 12409 25 0.0 12409 26 0.0 12409 27 0.0 12409 28 0.0 12409 29 0.0 12409 30 0.0 12409 31 0.0 12409 32 0.0 12409 33 0.0 12409 34 0.0 12409 35 0.0 12409 36 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 28 1.0 29 0.0 30 0.0 31 0.0 32 1.0 33 3.0 34 4.0 35 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.36363636363637 36.36363636363637 18.181818181818183 9.090909090909092 2 0.0 36.36363636363637 63.63636363636363 0.0 3 9.090909090909092 27.27272727272727 45.45454545454545 18.181818181818183 4 9.090909090909092 36.36363636363637 36.36363636363637 18.181818181818183 5 0.0 54.54545454545454 45.45454545454545 0.0 6 36.36363636363637 45.45454545454545 18.181818181818183 0.0 7 9.090909090909092 45.45454545454545 18.181818181818183 27.27272727272727 8 18.181818181818183 45.45454545454545 18.181818181818183 18.181818181818183 9 18.181818181818183 27.27272727272727 27.27272727272727 27.27272727272727 10 18.181818181818183 18.181818181818183 36.36363636363637 27.27272727272727 11 63.63636363636363 18.181818181818183 9.090909090909092 9.090909090909092 12 9.090909090909092 45.45454545454545 36.36363636363637 9.090909090909092 13 18.181818181818183 45.45454545454545 36.36363636363637 0.0 14 9.090909090909092 27.27272727272727 36.36363636363637 27.27272727272727 15 18.181818181818183 63.63636363636363 18.181818181818183 0.0 16 18.181818181818183 72.72727272727273 0.0 9.090909090909092 17 36.36363636363637 18.181818181818183 45.45454545454545 0.0 18 18.181818181818183 18.181818181818183 54.54545454545454 9.090909090909092 19 27.27272727272727 45.45454545454545 18.181818181818183 9.090909090909092 20 18.181818181818183 54.54545454545454 27.27272727272727 0.0 21 27.27272727272727 63.63636363636363 0.0 9.090909090909092 22 9.090909090909092 36.36363636363637 54.54545454545454 0.0 23 9.090909090909092 63.63636363636363 9.090909090909092 18.181818181818183 24 9.090909090909092 45.45454545454545 36.36363636363637 9.090909090909092 25 27.27272727272727 18.181818181818183 45.45454545454545 9.090909090909092 26 45.45454545454545 27.27272727272727 27.27272727272727 0.0 27 0.0 36.36363636363637 45.45454545454545 18.181818181818183 28 18.181818181818183 36.36363636363637 36.36363636363637 9.090909090909092 29 45.45454545454545 18.181818181818183 27.27272727272727 9.090909090909092 30 27.27272727272727 36.36363636363637 18.181818181818183 18.181818181818183 31 18.181818181818183 27.27272727272727 36.36363636363637 18.181818181818183 32 27.27272727272727 36.36363636363637 27.27272727272727 9.090909090909092 33 36.36363636363637 18.181818181818183 27.27272727272727 18.181818181818183 34 54.54545454545454 27.27272727272727 9.090909090909092 9.090909090909092 35 45.45454545454545 9.090909090909092 27.27272727272727 18.181818181818183 36 9.090909090909092 36.36363636363637 36.36363636363637 18.181818181818183 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.5 22 1.0 23 1.0 24 0.5 25 0.0 26 1.5 27 3.0 28 3.0 29 2.0 30 1.0 31 1.0 32 0.5 33 0.0 34 0.0 35 2.0 36 4.0 37 4.0 38 2.0 39 0.0 40 0.5 41 1.0 42 1.0 43 0.5 44 0.0 45 0.0 46 0.5 47 1.0 48 1.0 49 0.5 50 0.0 51 0.0 52 0.0 53 0.0 54 0.0 55 0.0 56 0.0 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 36 11.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATACATGACTGAGTGATTGTAGTTGGTAATACTGGA 1 9.090909090909092 No Hit ATCAAGAAGGGAGGAAGATGGTGATGAAGATGGGGA 1 9.090909090909092 No Hit ATTATATCCAATAATGAAGAGAAATTTAGAAACAGA 1 9.090909090909092 No Hit TATTAGCATCGTTCAATCCTCAATTATTTGCTGGCA 1 9.090909090909092 No Hit GTTCTGCAACATTTAAATAAAAAAATTCCATGCACT 1 9.090909090909092 No Hit GTATTTATCCTATCAAGTAGATCAGGAGGGCTAGGT 1 9.090909090909092 No Hit AAGAAAAGATGGTAAATGAAATAGGAAATCAAGGAG 1 9.090909090909092 No Hit CATGAGAGTAGCAAACGTAAGTCTAAAGGTTGTTTT 1 9.090909090909092 No Hit TTCTTACCATGAATTATTGAATAATGCTACTAACGT 1 9.090909090909092 No Hit GTTTAAATGGCAACAATGAAATACCGTTGAGTGATC 1 9.090909090909092 No Hit GAAATATATTGTATGGGTTTAAATTTCTTGGATGTC 1 9.090909090909092 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE