FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7425125
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG15850.011.9260231
TAGGTAG9800.010.2556255
TGGGGGG8300.010.1209975
TATTAGG16250.09.6004892
CTAATAC17000.09.2651783
TCTATAC16900.09.1424783
TAGGGGG4300.09.07023054
CTATACT18350.08.747044
GTATAGA19100.08.6399981
CCTAATA18050.08.5599942
TAGACTG8450.08.5211455
CTGCGCG2505.6170775E-68.3992979
ACTATGC17650.08.3279158
AGGGGGG8650.08.3241235
CCTACAC6850.08.3215923
CTAGGAC5800.08.2762843
TAATACT26200.08.187444
TACTATG19900.08.1399687
GTATTAA17200.08.1116441
CCCTACA5950.08.0676382
Produced by FastQC (version 0.11.2)