FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122975
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCGG452.0417692E-523.33156613
GTCGGGG553.8875005E-621.8165315
TAGTCAG2050.017.5596479
TAGGTAG2350.015.9590825
GTATTAG2950.015.7664331
TAGACTG1206.8619556E-815.0015355
CGTAGAG2200.015.00086928
TCCCCCA600.003752404615.00086827
TGCGCGC706.0401746E-414.99886520
ACAGGCG600.00375574114.9988648
GACGTAG2300.014.34865726
TCCGAGC2200.014.31709813
CCGAGCC2200.014.31709814
GAGCCCA2350.014.04149116
TCGGGGG750.001018502913.9989416
CCGTCGC1301.8303945E-713.8451069
GTCCCGG650.00638912913.8451069
AGCCCAC2400.013.74895917
GTAGTCA2750.013.6353318
GCGCACT902.65356E-413.33232411
Produced by FastQC (version 0.11.2)