FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2235736
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG2300.013.0440324
TAGGGGG1951.1659722E-912.3082154
ATCGTGC1601.7171624E-712.1863828
GTATTAG5700.011.8436681
TAGGTAG4100.011.7078145
GGTAGTC2950.011.6938437
TACACTG4000.011.2504785
AGGGGGG2701.2732926E-1111.1115835
CTAGGAC1501.3601819E-511.0004683
GCCGTGC1100.001434698710.9080918
CCGTGTA1253.4269743E-410.799019
CAGCGTC2701.546141E-1010.556242
ACTGGTC2352.1698725E-810.211838
TATTAGG7100.09.9302222
CTAATAC7150.09.86055853
TTAGGGG2301.7284947E-79.7830243
GCCTTAC2157.236704E-79.7687311
CGTGGGC1250.00414597949.599128
TCTAGGA3001.0513759E-99.5006172
TAATACT10750.09.4887754
Produced by FastQC (version 0.11.2)