FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1526892
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGC455.469379E-419.9990838
CTAGGAC901.581443E-515.0007863
TAGGGGG952.634951E-514.2112714
TGGGGGG1507.381277E-813.0006825
GTATTAG3550.012.2557691
ACTGCGC1006.453593E-411.9994518
GTATAAT5900.011.95134451
CGCCGCG1151.5787611E-411.73936124
ACAGTCC1806.268965E-811.6661328
ACTGGTC1602.2635777E-611.2494848
TAATACT7150.010.6998624
TAGACAG2402.6502676E-910.6255575
TTAGACA4000.010.500554
GGACCCA1000.00877588710.500556
GCCGCGT1304.913929E-410.385159525
TAGGTAG1451.1663643E-410.345375
TACTCCG1451.1663643E-410.345375
TACAGCG2505.1968527E-910.1995327
GTATAGA3750.010.0018341
AGGGGGG1501.6429104E-410.00052455
Produced by FastQC (version 0.11.2)