FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041615
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG1356.184564E-1116.668245
ACCCCGT550.00210283716.3620410
CTAGCCT706.0318614E-415.0014164
GCGCACT901.5827289E-514.99853611
CGCACTC750.001018584513.99863312
TAGTCAG1454.7401045E-813.4469639
GGTAGTC1404.5129673E-712.8552717
TGGGGGG1456.885675E-712.4149665
GTATTAC2001.7298589E-912.0022851
TATACAG4400.011.9329455
GTGTCCT900.00400957611.6655289
CTGATCC900.00400957611.6655289
TAAGTAG1551.5407859E-611.6139995
ATTATAC4150.011.5673573
ACTACAC1951.5274054E-811.5395513
TATACTG4300.011.5127155
TGTGTCC1059.717395E-411.4274568
CTTATCC2155.3896656E-911.1638453
GTACCGT950.00599175211.0536756
ACTGTAG950.00600032211.0515538
Produced by FastQC (version 0.11.2)