FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1658359
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG2203.6379788E-1212.9546495
GTCTTAG1753.0686351E-912.858411
CCGAGCC954.1910037E-412.630918514
TATACCG3100.012.5807465
TAATACT6800.012.1324514
CCCTTAT3750.012.0011831
CTAATAC5050.011.8812843
CTACGCA900.004009928611.6660579
TGGGGGG2103.741661E-911.4286645
TATACTG6400.011.250095
GTATTAG4550.011.2098951
TACACTG2751.8189894E-1110.9091795
TATACAC4550.010.87920953
CTACACA2802.7284841E-1110.7143724
TAAGGCT2701.546141E-1010.555644
CGGGGTC1000.00878323710.49945113
CCTGGGC1000.00878323710.4994518
GCGTGGG1000.00878323710.49945111
GCGCCGA1150.002074403710.43549626
CAGTCTG1150.002076485410.4342379
Produced by FastQC (version 0.11.2)