FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1280600
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAAG752.793633E-618.0020621
TGGGGGG957.9293386E-817.3683785
TAGACCG2101.8189894E-1213.5713935
CGACGGG1004.2744934E-513.498918
CGGGCGG1004.2744934E-513.4989111
AACAGTC1855.0749804E-1012.9719267
TCTATAC2700.012.7777453
CGGGTCC1302.7567494E-612.69326625
GTCGCTT1551.1339398E-712.5796311
GGTTAGG1201.691619E-512.5014311
GGACTAC1252.5742436E-511.9999696
TCTGTAC1406.315653E-611.7856853
TGTACCT1406.315653E-611.7856855
CGGCCCC900.004005250511.66754725
CGACCCG900.00400758811.66663616
CCGTCGC1806.2684194E-811.6657259
ACCGTCG2452.5465852E-1111.6317158
GTCTAGT1303.8397942E-511.53978351
ACAGTGC1303.847458E-511.5375318
GTGTAGG1059.7028003E-411.4298811
Produced by FastQC (version 0.11.2)