FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3475835
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2950.012.204155
GTATTAG9600.011.7199871
TAGGGGG2052.564775E-911.7080474
TGGACTG3550.011.40916255
TAGGTAG4300.011.16348655
GACAGCG1901.3278441E-711.0520487
TAGACAG5450.011.009865
ACGGTGT7050.010.2120818
ACTGCGC2505.2114046E-910.1993158
TTATACT14050.010.1429814
CGCGCCT1501.645071E-410.00004922
TATACGG9700.09.8975245
ACTATGC9450.09.8406098
TATACTG14050.09.8226765
CTAAGAC3700.09.7301973
CGCGGCC1409.724496E-49.64234813
GACCCGC1250.0041461199.59935611
TATACAG19000.09.5532275
GTATAAT15400.09.5464621
TAGGACC3306.002665E-119.5460494
Produced by FastQC (version 0.11.2)