FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2874192
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG3050.013.2801135
GTCCTAA3650.012.7424241
TAGGGGT2351.2732926E-1112.1289614
TCCCGCG900.00401158411.6658897
TAGGTAG3950.011.0138415
TCTATAC7100.010.9870943
CCGCGCG1100.001434615710.9083629
AGGGGGG2905.456968E-1210.86323455
TCTAGGG2504.5292836E-1010.8011593
TTCTAGG4600.010.7618372
GTACTAG3500.010.7165541
CCTGGAC2104.5660272E-810.7154363
CCCGCGC1408.1885315E-510.7135728
GTATTAG5950.010.5904781
CCCTAAG1851.1189331E-610.5427721
CGCGCAT1304.9229484E-410.38374210
CTAAGGC3350.010.2996123
GGCACGC1756.626333E-610.2848512
CTAGACT2053.7721293E-710.2450024
ATCCGCG1501.6460326E-49.9993337
Produced by FastQC (version 0.11.2)