FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309559
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG400.006511352518.749815
GCAGTGC500.001110695917.999138
TGGGGGG1206.874507E-814.9998465
GTATTAG4200.013.2156661
TAGGTAG1855.0749804E-1012.9728415
TACACCG1201.6938571E-512.4998725
GGTGACC1059.7163033E-411.4280199
GCTCAGC1059.7163033E-411.42801912
CATACTG2259.113137E-1011.3332185
CCCGAAA950.005999519511.05209713
GCGTCAG1501.3597844E-510.9998884
TTAGGAC1653.2720727E-610.9089793
GTATATA14350.010.7676741
GGTCGTG1000.00878132310.4994937
TAGGCTG1150.002075256810.4346765
ACTGCGC1150.002075915710.43427858
ATACAGG5500.010.363536
TTAGGTA3051.2732926E-1110.3277644
GCAGCGT1602.7714837E-510.3135761
TATACCG3203.6379788E-1210.3123945
Produced by FastQC (version 0.11.2)