##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	HK5NHBGXX_combined_n01_ntr_3.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26
Sequences flagged as poor quality	0
Sequence length	36
%GC	38
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.192307692307693	0.0	0.0	0.0	0.0	0.0
2	30.884615384615383	0.0	0.0	0.0	0.0	0.0
3	31.307692307692307	0.0	0.0	0.0	0.0	0.0
4	31.307692307692307	0.0	0.0	0.0	0.0	0.0
5	31.307692307692307	0.0	0.0	0.0	0.0	0.0
6	32.42307692307692	0.0	0.0	0.0	0.0	0.0
7	34.30769230769231	0.0	0.0	0.0	0.0	0.0
8	33.53846153846154	0.0	0.0	0.0	0.0	0.0
9	35.69230769230769	0.0	0.0	0.0	0.0	0.0
10	35.0	0.0	0.0	0.0	0.0	0.0
11	33.84615384615385	0.0	0.0	0.0	0.0	0.0
12	35.26923076923077	0.0	0.0	0.0	0.0	0.0
13	32.69230769230769	0.0	0.0	0.0	0.0	0.0
14	35.53846153846154	0.0	0.0	0.0	0.0	0.0
15	33.46153846153846	0.0	0.0	0.0	0.0	0.0
16	34.07692307692308	0.0	0.0	0.0	0.0	0.0
17	34.53846153846154	0.0	0.0	0.0	0.0	0.0
18	34.0	0.0	0.0	0.0	0.0	0.0
19	35.0	0.0	0.0	0.0	0.0	0.0
20	33.65384615384615	0.0	0.0	0.0	0.0	0.0
21	34.69230769230769	0.0	0.0	0.0	0.0	0.0
22	32.30769230769231	0.0	0.0	0.0	0.0	0.0
23	33.61538461538461	0.0	0.0	0.0	0.0	0.0
24	34.42307692307692	0.0	0.0	0.0	0.0	0.0
25	35.53846153846154	0.0	0.0	0.0	0.0	0.0
26	33.15384615384615	0.0	0.0	0.0	0.0	0.0
27	34.0	0.0	0.0	0.0	0.0	0.0
28	34.0	0.0	0.0	0.0	0.0	0.0
29	32.5	0.0	0.0	0.0	0.0	0.0
30	35.0	0.0	0.0	0.0	0.0	0.0
31	33.96153846153846	0.0	0.0	0.0	0.0	0.0
32	34.84615384615385	0.0	0.0	0.0	0.0	0.0
33	33.26923076923077	0.0	0.0	0.0	0.0	0.0
34	32.46153846153846	0.0	0.0	0.0	0.0	0.0
35	34.84615384615385	0.0	0.0	0.0	0.0	0.0
36	34.15384615384615	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
12602	1	0.0
12602	2	-3.5
12602	3	0.0
12602	4	0.0
12602	5	0.0
12602	6	-7.5
12602	7	0.0
12602	8	-9.0
12602	9	2.0
12602	10	2.0
12602	11	0.0
12602	12	0.0
12602	13	11.0
12602	14	0.0
12602	15	-11.0
12602	16	2.0
12602	17	0.0
12602	18	0.0
12602	19	0.0
12602	20	0.0
12602	21	0.0
12602	22	-11.0
12602	23	0.0
12602	24	0.0
12602	25	0.0
12602	26	0.0
12602	27	-2.0
12602	28	11.0
12602	29	3.5
12602	30	0.0
12602	31	-11.0
12602	32	2.0
12602	33	0.0
12602	34	-11.0
12602	35	-2.0
12602	36	0.0
13202	1	0.0
13202	2	3.5
13202	3	0.0
13202	4	0.0
13202	5	0.0
13202	6	7.5
13202	7	0.0
13202	8	9.0
13202	9	-2.0
13202	10	-2.0
13202	11	0.0
13202	12	0.0
13202	13	-11.0
13202	14	0.0
13202	15	11.0
13202	16	-2.0
13202	17	0.0
13202	18	0.0
13202	19	0.0
13202	20	0.0
13202	21	0.0
13202	22	11.0
13202	23	0.0
13202	24	0.0
13202	25	0.0
13202	26	0.0
13202	27	2.0
13202	28	-11.0
13202	29	-3.5
13202	30	0.0
13202	31	11.0
13202	32	-2.0
13202	33	0.0
13202	34	11.0
13202	35	2.0
13202	36	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
26	1.0
27	0.0
28	0.0
29	0.0
30	1.0
31	1.0
32	3.0
33	5.0
34	10.0
35	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.30769230769231	15.384615384615385	19.230769230769234	23.076923076923077
2	3.8461538461538463	26.923076923076923	57.692307692307686	11.538461538461538
3	11.538461538461538	26.923076923076923	34.61538461538461	26.923076923076923
4	23.076923076923077	11.538461538461538	26.923076923076923	38.46153846153847
5	11.538461538461538	34.61538461538461	46.15384615384615	7.6923076923076925
6	19.230769230769234	57.692307692307686	11.538461538461538	11.538461538461538
7	34.61538461538461	23.076923076923077	38.46153846153847	3.8461538461538463
8	15.384615384615385	57.692307692307686	19.230769230769234	7.6923076923076925
9	38.46153846153847	3.8461538461538463	26.923076923076923	30.76923076923077
10	15.384615384615385	30.76923076923077	46.15384615384615	7.6923076923076925
11	34.61538461538461	38.46153846153847	26.923076923076923	0.0
12	26.923076923076923	7.6923076923076925	42.30769230769231	23.076923076923077
13	11.538461538461538	38.46153846153847	11.538461538461538	38.46153846153847
14	15.384615384615385	19.230769230769234	42.30769230769231	23.076923076923077
15	26.923076923076923	38.46153846153847	15.384615384615385	19.230769230769234
16	15.384615384615385	57.692307692307686	19.230769230769234	7.6923076923076925
17	19.230769230769234	26.923076923076923	34.61538461538461	19.230769230769234
18	15.384615384615385	38.46153846153847	19.230769230769234	26.923076923076923
19	19.230769230769234	26.923076923076923	26.923076923076923	26.923076923076923
20	30.76923076923077	15.384615384615385	42.30769230769231	11.538461538461538
21	15.384615384615385	23.076923076923077	38.46153846153847	23.076923076923077
22	0.0	38.46153846153847	23.076923076923077	38.46153846153847
23	19.230769230769234	46.15384615384615	15.384615384615385	19.230769230769234
24	15.384615384615385	26.923076923076923	26.923076923076923	30.76923076923077
25	19.230769230769234	26.923076923076923	38.46153846153847	15.384615384615385
26	23.076923076923077	26.923076923076923	38.46153846153847	11.538461538461538
27	11.538461538461538	30.76923076923077	38.46153846153847	19.230769230769234
28	7.6923076923076925	15.384615384615385	42.30769230769231	34.61538461538461
29	19.230769230769234	30.76923076923077	34.61538461538461	15.384615384615385
30	15.384615384615385	46.15384615384615	19.230769230769234	19.230769230769234
31	26.923076923076923	30.76923076923077	34.61538461538461	7.6923076923076925
32	15.384615384615385	19.230769230769234	38.46153846153847	26.923076923076923
33	11.538461538461538	23.076923076923077	46.15384615384615	19.230769230769234
34	7.6923076923076925	11.538461538461538	38.46153846153847	42.30769230769231
35	19.230769230769234	23.076923076923077	38.46153846153847	19.230769230769234
36	11.538461538461538	30.76923076923077	38.46153846153847	19.230769230769234
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.5
25	1.0
26	2.0
27	3.0
28	3.0
29	2.0
30	1.0
31	1.0
32	2.0
33	3.0
34	3.0
35	5.5
36	8.0
37	8.0
38	5.0
39	2.0
40	2.0
41	2.0
42	2.0
43	1.5
44	1.0
45	1.0
46	1.0
47	1.0
48	1.0
49	1.0
50	1.0
51	0.5
52	0.0
53	0.0
54	0.5
55	1.0
56	1.0
57	1.0
58	1.0
59	1.0
60	0.5
61	0.0
62	0.0
63	0.5
64	1.0
65	0.5
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
36	26.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.15384615384616
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.0	92.3076923076923
2	4.0	7.6923076923076925
3	0.0	0.0
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATTCTAGAGGACATGAACAGCAACTTACAAGTCTTA	2	7.6923076923076925	No Hit
GTACAATGATGACGCATAGTTCACGATATGTTTCTT	1	3.8461538461538463	No Hit
GATATCAATATCCCTACTATCAACAGCGTAACTTGG	1	3.8461538461538463	No Hit
GCTGTCGATATTGGGGAACTGTGGTGGTTGGCAAAT	1	3.8461538461538463	No Hit
TCCTCAGACTTTCTGATGTCAAATCTTCTAAATTGA	1	3.8461538461538463	No Hit
CTTGAAGATGAGACAAGATTGATCGGTTCATGGCAA	1	3.8461538461538463	No Hit
GTTCAAGATTTGCCTTATTCTTCTCTATGCTCTTCT	1	3.8461538461538463	No Hit
CACCATACCATGCACTTACTTAAATACTATGAACAC	1	3.8461538461538463	No Hit
TACTTCTTTAGCGCAAGGGGTAGGATCGATCCACTT	1	3.8461538461538463	No Hit
GTGCCAGGGTATCTAGCACGCTCTGCTCAAAAGTCC	1	3.8461538461538463	No Hit
TTCCATCACAGCACATTTGATTTCATTTCTGTCGTA	1	3.8461538461538463	No Hit
GACAGGTACAATCATATTCTTCATAGTCAGTTTTCT	1	3.8461538461538463	No Hit
GTCTTAAAGATTTGAAAAGGTCAACACCGATTTCAC	1	3.8461538461538463	No Hit
ATTGGGTAGTGGACAACCAGACAATGACACTGTTGT	1	3.8461538461538463	No Hit
CAAGTAGTCCGCCTAGCAGAGCACGCCCCACCAAGC	1	3.8461538461538463	No Hit
CTATAATGCAGTCTCTTGATAACTTTTTGCACTGTA	1	3.8461538461538463	No Hit
GTTATATAGTAGAAAGGATGAAGAAAATTTATTCCG	1	3.8461538461538463	No Hit
GTGTAGACTTGAGTGACCATTTCATCTTCAATACGA	1	3.8461538461538463	No Hit
CTATTGTATCATTTGATACACCAAAAGTGAAATCAT	1	3.8461538461538463	No Hit
GAACAAAAGGGTAAAAGCCCCCTTGATTTTGATTTT	1	3.8461538461538463	No Hit
TTCGAATGCTATATCCTCAGTAGCTTAATATTAATC	1	3.8461538461538463	No Hit
ACTCTGTTGTGGCACCGCTAACAGTAACGGTGGCCG	1	3.8461538461538463	No Hit
CAGCTAATGTTGATGAATCTTCCGCGGAAAACTCTT	1	3.8461538461538463	No Hit
GTAGGATTGTATTTTATGTTATGAATATTCGTCCTA	1	3.8461538461538463	No Hit
TGATTTGACTATAGATAATTGCTTTTTATCTGCTAT	1	3.8461538461538463	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10	0.0	0.0	0.0
11	0.0	0.0	0.0
12	0.0	0.0	0.0
13	0.0	0.0	0.0
14	0.0	0.0	0.0
15	0.0	0.0	0.0
16	0.0	0.0	0.0
17	0.0	0.0	0.0
18	0.0	0.0	0.0
19	0.0	0.0	0.0
20	0.0	0.0	0.0
21	0.0	0.0	0.0
22	0.0	0.0	0.0
23	0.0	0.0	0.0
24	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE